Article
Quantification of microbial uptake of quercetin and its derivatives using an UHPLC-ESI-QTOF mass spectrometry assay.
Food & function - 14 Sept 2016
Said Inamullah Hakeem, Shah Rohan Lakshmidas, Ullrich Matthias S, Kuhnert Nikolai
Abstract excerpt
A liquid chromatography-mass spectrometry-based method for the quantification of intracellular concentrations of dietary phenolics in bacteria was developed. Using this assay, the time-dependent uptake of quercetin and two of its glycosides into gut microbiota model organisms (Escherichia coli and Bifidobacterium bifidum) under aerobic and anaerobic conditions was studied. For the first time, quantitative data on...
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