Article
Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins.
Journal of molecular biology - 22 Jul 2005
Drummond D Allan, Iverson Brent L, Georgiou George, Arnold Frances H
Abstract excerpt
The fraction of proteins that retain wild-type function after mutation has long been observed to decline exponentially as the average number of mutations per gene increases. Recently, several groups have used error-prone polymerase chain reactions (PCR) to generate libraries with 15 to 30 mutatio...
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