Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins.

Abstract excerpt

The fraction of proteins that retain wild-type function after mutation has long been observed to decline exponentially as the average number of mutations per gene increases. Recently, several groups have used error-prone polymerase chain reactions (PCR) to generate libraries with 15 to 30 mutations per gene, on average, and have reported that orders of magnitude more proteins retain function than would be...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Data Interpretation, Statistical
• Share to Gene Library