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Article

High Throughput Random Mutagenesis and Single Molecule Real Time Sequencing of the Muscle Nicotinic Acetylcholine Receptor.

PloS one - 1 Jan 2016

Groot-Kormelink Paul J, Ferrand Sandrine, Kelley Nicholas, Bill Anke, Freuler Felix, Imbert Pierre-Eloi, Marelli Anthony, Gerwin Nicole, Sivilotti Lucia G, Miraglia Loren, Orth Anthony P, Oakeley Edward J, Schopfer Ulrich, Siehler Sandra

Abstract excerpt

High throughput random mutagenesis is a powerful tool to identify which residues are important for the function of a protein, and gain insight into its structure-function relation. The human muscle nicotinic acetylcholine receptor was used to test whether this technique previously used for monomeric receptors can be applied to a pentameric ligand-gated ion channel. A mutant library for the α1 subunit of the...

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PMID: 27649498
DOI: 10.1371/journal.pone.0163129
PMCID: PMC5029940

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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