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Article

Direct assay for endo-α-mannosidase substrate preference on correctly folded and misfolded model glycoproteins.

Carbohydrate research - 3 Nov 2016

Dedola Simone, Izumi Masayuki, Makimura Yutaka, Seko Akira, Kanamori Akiko, Takeda Yoichi, Ito Yukishige, Kajihara Yasuhiro

Abstract excerpt

We previously reported a unique assay system for UDP-glucose glycoprotein glucosyltransferase (UGGT) toward glycoprotein folding intermediates during the folding process. The assay involved the in vitro folding of both high-mannose type oligosaccharyl crambin, which yielded only the correctly folded glycoprotein form (M9-glycosyl-native-crambin), and its mutant, which yielded misfolded glycoproteins...

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Identifiers

PMID: 27623439
DOI: 10.1016/j.carres.2016.08.008

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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