Article
Transglycosylation Activity of Glycosynthase Mutants of Endo-β-N-Acetylglucosaminidase from Coprinopsis cinerea.
PloS one - 1 Jan 2015
Eshima Yasunari, Higuchi Yujiro, Kinoshita Takashi, Nakakita Shin-Ichi, Takegawa Kaoru
Abstract excerpt
Endo-β-N-acetylglucosaminidase (ENGase), which catalyzes hydrolysis of N-linked oligosaccharides, is a useful tool for analyzing oligosaccharide contents of glycoproteins. However, there are only a few known ENGases that can catalyze the hydrolysis of human complex type oligosaccharides, and although commercially available, they are expensive. Here, we report the cloning of two ENGase encoding cDNAs from the...
Topics
- Acetylglucosaminidase
- Basidiomycota
- Catalysis
- Cloning, Molecular
- DNA, Complementary
- Escherichia coli
- Glycoproteins
- Glycosylation
- Humans
- Hydrolases
- Hydrolysis
- Mannose
- Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase
- Mutation