CRISPR screens in plants: approaches, guidelines, and future prospects.

Abstract excerpt

Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems have revolutionized genome engineering by facilitating a wide range of targeted DNA perturbations. These systems have resulted in the development of powerful new screens to test gene functions at the genomic scale. While there is tremendous potential to map and interrogate gene regulatory networks at unprecedented speed and scale...

Topics

• CRISPR-Cas Systems
• Clustered Regularly Interspaced Short Palindromic Repeats
• Gene Editing
• Gene Knockout Techniques
• High-Throughput Screening Assays
• Mutation
• Plants
• Plants, Genetically Modified
• Population Density
• RNA, Guide, CRISPR-Cas Systems