Article
Microparticle-based RT-qPCR for highly selective rare mutation detection.
Biosensors & bioelectronics - 15 Jan 2017
Oh Eun Hae, Jung Seungwon, Kim Won Jin, Kim Kwang Pyo, Kim Sang Kyung
Abstract excerpt
The quantitative reverse transcription polymerase chain reaction (RT-qPCR) has become one of the most widely used methods in the detection of disease-specific RNAs. The RT-qPCR involves two separate steps, RT and qPCR. In this study, we suggest a new RT-qPCR protocol with the particles of primer-immobilized networks (PINs), performing capture, RT and amplification of a target RNA in one particle. The production...
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