Article
A single aspartate mutation in the conserved catalytic site of Rev3L generates a hypomorphic phenotype in vivo and in vitro.
DNA repair - 1 Oct 2016
Fritzen Rémi, Delbos Frédéric, De Smet Annie, Palancade Benoît, Canman Christine E, Aoufouchi Said, Weill Jean-Claude, Reynaud Claude-Agnès, Storck Sébastien
Abstract excerpt
Rev3, the catalytic subunit of yeast DNA polymerase ζ, is required for UV resistance and UV-induced mutagenesis, while its mammalian ortholog, REV3L, plays further vital roles in cell proliferation and embryonic development. To assess the contribution of REV3L catalytic activity to its in vivo function, we generated mutant mouse strains in which one or two Ala residues were substituted to the Asp of the invariant...
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