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Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization.

Molecular cell - 4 Aug 2016

Rodríguez-Molina Juan B, Tseng Sandra C, Simonett Shane P, Taunton Jack, Ansari Aseem Z

Abstract excerpt

During transcription initiation, the TFIIH-kinase Kin28/Cdk7 marks RNA polymerase II (Pol II) by phosphorylating the C-terminal domain (CTD) of its largest subunit. Here we describe a structure-guided chemical approach to covalently and specifically inactivate Kin28 kinase activity in vivo. This method of irreversible inactivation recapitulates both the lethal phenotype and the key molecular signatures that...

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Identifiers

PMID: 27477907
DOI: 10.1016/j.molcel.2016.06.036
PMCID: PMC5122673

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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