Article
The effect of growth factor supplementation on corneal stromal cell phenotype in vitro using a serum-free media.
Experimental eye research - 1 Oct 2016
Lynch Amy P, O'Sullivan Finbarr, Ahearne Mark
Abstract excerpt
In order to expand cells quickly and in high numbers for corneal tissue engineering applications corneal stromal cells, or keratocytes, are often cultured in the presence of serum. However, keratocytes become fibroblastic when exposed to serum leading to a downregulation of corneal stromal specific markers. The purpose of this current study was to determine if corneal stromal cells, made fibroblastic by serum,...
Topics
- Adult
- Blotting, Western
- Cell Movement
- Cell Proliferation
- Cells, Cultured
- Corneal Keratocytes
- Corneal Stroma
- Culture Media, Serum-Free
- DNA
- Extracellular Matrix Proteins
- Gene Expression Regulation
- Humans
- Phenotype
- Real-Time Polymerase Chain Reaction
- Tissue Engineering
- Transforming Growth Factor beta1