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Preservation and expansion of the primate keratocyte phenotype by downregulating TGF-beta signaling in a low-calcium, serum-free medium.

Investigative ophthalmology & visual science - 1 May 2006

Kawakita Tetsuya, Espana Edgar M, He Hua, Smiddy Robert, Parel Jean-Marie, Liu Chia-Yang, Tseng Scheffer C G

Abstract excerpt

PURPOSE: To demonstrate whether the original keratocyte phenotype is maintained with proliferative activity by suppressing TGF-beta signaling in rhesus monkey keratocytes expanded in a serum-free and low-[Ca2+] medium. METHODS: Rhesus monkey keratocytes were isolated from central corneal buttons by collagenase digestion for 16 hours, seeded on plastic in Dulbecco's modified Eagle's medium (DMEM) containing...

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Identifiers

PMID: 16638999
DOI: 10.1167/iovs.05-1040
PMCID: PMC1569676

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Source receipt: pubmed, retrieved 7 Sept 2026.

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