Article
Analysis of carotenoid profile changes and carotenogenic genes transcript levels in Rhodosporidium toruloides mutants from an optimized Agrobacterium tumefaciens-mediated transformation method.
Biotechnology and applied biochemistry - 1 Feb 2021
Sun Zeping, Lv Jing, Ji Chaofan, Liang Huipeng, Li Shengjie, Yang Zhaoxia, Xu Wenhuan, Zhang Sufang, Lin Xinping
Abstract excerpt
Rhodosporidium toruloides has been reported as a potential biotechnological microorganism to produce carotenoids. The most commonly used molecular and genetic manipulation methods based on Agrobacterium-mediated transformation (ATMT). However, this method was of relatively lower transformation efficiency. In this study, we optimized the ATMT method for R. toruloides on account of the promoter on T-DNA, the ratio...
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