Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering.

Nucleic acids research - 8 Jul 2016

Bendl Jaroslav, Stourac Jan, Sebestova Eva, Vavra Ondrej, Musil Milos, Brezovsky Jan, Damborsky Jiri

Abstract excerpt

HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart libraries for engineering proteins' stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evolutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Automation
Share to Biocatalysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Databases, Protein

Share to Internet

Share to Peptide Library

Share to Protein Stability

Share to Software

Share to Substrate Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 27174934
DOI: 10.1093/nar/gkw416
PMCID: PMC4987947

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering.PMID 27174934Read ↗

HotSpot Wizard 3.0: web server for automated design of mutations and smart libraries based on sequence input information.Semantic proximity Read ↗

Hot spots-making directed evolution easier.Semantic proximity Read ↗

Hot spots in protein-protein interfaces: towards drug discovery.Semantic proximity Read ↗

Techniques for Developing Reliable Machine Learning Classifiers Applied to Understanding and Predicting Protein:Protein Interaction Hot SpotsSemantic proximity Read ↗

AutoMatch: target-binding protein design and enzyme design by automatic pinpointing potential active sites in available protein scaffolds.Semantic proximity Read ↗

FireProt 2.0: web-based platform for the fully automated design of thermostable proteins.Semantic proximity Read ↗

PIIMS Server: A Web Server for Mutation Hotspot Scanning at the Protein-Protein Interface.Semantic proximity Read ↗

FireProt: web server for automated design of thermostable proteins.Semantic proximity Read ↗

The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering.Semantic proximity Read ↗

Designed active-site library reveals thousands of functional GFP variants.Semantic proximity Read ↗

Categorization of hotspots into three types - weak, moderate and strong to distinguish protein-protein versus protein-peptide interactions.Semantic proximity Read ↗

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.