Article
MRPrimerW: a tool for rapid design of valid high-quality primers for multiple target qPCR experiments.
Nucleic acids research - 8 Jul 2016
Kim Hyerin, Kang NaNa, An KyuHyeon, Koo JaeHyung, Kim Min-Soo
Abstract excerpt
Design of high-quality primers for multiple target sequences is essential for qPCR experiments, but is challenging due to the need to consider both homology tests on off-target sequences and the same stringent filtering constraints on the primers. Existing web servers for primer design have major drawbacks, including requiring the use of BLAST-like tools for homology tests, lack of support for ranking of primers,...
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