Article

MultiPrime: A Reliable and Efficient Tool for Targeted Next-Generation Sequencing

2023-06-30

Abstract excerpt

<title>Abstract</title> <p>We present multiPrime, a novel tool that automatically designs minimal primer sets for target next generation sequencing. It enhances coverage by designing primers with mismatch tolerance, ensuring high compatibility and specificity. We compared the performance of multiPrime to that of conventional tools using a dataset of 43,016 sequences from eight viruses. Our results demonstrate tha...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 1176d1b9-ca74-53b4-a357-a3a7f5761967
DOI: 10.21203/rs.3.rs-2893323/v3

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

MultiPrime: A Reliable and Efficient Tool for Targeted Next-Generation SequencingDOI 10.21203/rs.3.rs-2893323/v3Read

CoVrimer: A tool for aligning SARS-CoV-2 primer sequences and selection of conserved/degenerate primers.Semantic proximity Read

varVAMP: automated pan-specific primer design for tiled full genome sequencing and qPCR of highly diverse viral pathogensSemantic proximity Read

varVAMP: degenerate primer design for tiled full genome sequencing and qPCRSemantic proximity Read

Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of AlignmentsSemantic proximity Read

primerJinn: a tool for rationally designing multiplex PCR primer sets for amplicon sequencing and performing in silico PCRSemantic proximity Read

URAdime – a tool for analyzing primer sequences in sequencing data to identify dimers and super-ampliconsSemantic proximity Read

PrimalScheme: open-source community resources for low-cost viral genome sequencingSemantic proximity Read

DeGenPrime provides robust primer design and optimization unlocking the biosphereSemantic proximity Read

Multiple-primer DNA sequencing method.Semantic proximity Read

primerJinn – a tool for rationally designing multiplex PCR primer sets and in silico PCRSemantic proximity Read

A targeted approach with nanopore sequencing for the universal detection and identification of flavivirusesSemantic proximity Read

A Computational Toolset for Rapid Identification of SARS-CoV-2, other Viruses, and Microorganisms from Sequencing DataSemantic proximity Read