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Article

Mutant DNA quantification by digital PCR can be confounded by heating during DNA fragmentation.

BioTechniques - 1 Apr 2016

Kang Qing, Parkin Brian, Giraldez Maria D, Tewari Muneesh

Abstract excerpt

Digital PCR (dPCR) is gaining popularity as a DNA mutation quantification method for clinical specimens. Fragmentation prior to dPCR is required for non-fragmented genomic DNA samples; however, the effect of fragmentation on DNA analysis has not been well-studied. Here we evaluated three fragmentation methods for their effects on dPCR point mutation assay performance. Wild-type (WT) human genomic DNA was...

Read the complete abstract on PubMed

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Share to DNA
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Dosage

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Share to Polymerase Chain Reaction

Share to Proto-Oncogene Proteins B-raf

Share to Proto-Oncogene Proteins p21(ras)

Source

Identifiers

PMID: 27071606
DOI: 10.2144/000114401

Genes

KRAS
BRAF

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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