Article
In Vivo Depletion of T Lymphocytes.
Current protocols in immunology - 1 Apr 2016
Laky Karen, Kruisbeek Ada M
Abstract excerpt
In vivo depletion of T lymphocytes is a means of studying the role of specific T cell populations during defined phases of in vivo immune responses. In this unit, a protocol is provided for injecting monoclonal antibodies (mAbs) into wild-type adult mice. Depletion of the appropriate subset of cells is verified by flow cytometry analysis of lymph node and spleen cell suspensions in pilot experiments. Once...
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