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Article

Uncoupling JAK2 V617F activation from cytokine-induced signalling by modulation of JH2 αC helix.

The Biochemical journal - 1 Jun 2016

Leroy Emilie, Dusa Alexandra, Colau Didier, Motamedi Amir, Cahu Xavier, Mouton Céline, Huang Lily J, Shiau Andrew K, Constantinescu Stefan N

Abstract excerpt

The mechanisms by which JAK2 is activated by the prevalent pseudokinase (JH2) V617F mutation in blood cancers remain elusive. Via structure-guided mutagenesis and transcriptional and functional assays, we identify a community of residues from the JH2 helix αC, SH2-JH2 linker and JH1 kinase domain that mediate V617F-induced activation. This circuit is broken by altering the charge of residues along the...

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Identifiers

PMID: 27029346
DOI: 10.1042/BCJ20160085
PMCID: PMC4888464

Genes

JAK2

Variants

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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