Substitution of pseudokinase domain residue Val-617 by large non-polar amino acids causes activation of JAK2.

Abstract excerpt

Explaining the uniqueness of the acquired somatic JAK2 V617F mutation, which is present in more than 95% of polycythemia vera patients, has been a challenge. The V617F mutation in the pseudokinase domain of JAK2 renders the unmutated kinase domain constitutively active. We have performed random mutagenesis at position 617 of JAK2 and tested each of the 20 possible amino acids for ability to induce constitutive...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Bone Marrow Transplantation
• Share to Gene Expression Regulation, Enzymologic