Article
Combining somatic mutations present in different in vivo affinity-matured antibodies isolated from immunized Lama glama yields ultra-potent antibody therapeutics.
Protein engineering, design & selection : PEDS - 1 Apr 2016
Klarenbeek Alex, Blanchetot Christophe, Schragel Georg, Sadi Ava S, Ongenae Nico, Hemrika Wieger, Wijdenes John, Spinelli Silvia, Desmyter Aline, Cambillau Christian, Hultberg Anna, Kretz-Rommel Anke, Dreier Torsten, De Haard Hans J W, Roovers Rob C
Abstract excerpt
Highly potent human antibodies are required to therapeutically neutralize cytokines such as interleukin-6 (IL-6) that is involved in many inflammatory diseases and malignancies. Although a number of mutagenesis approaches exist to perform antibody affinity maturation, these may cause antibody instability and production issues. Thus, a robust and easy antibody affinity maturation strategy to increase antibody...
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