Article
Initiation on the divergent Type I cadicivirus IRES: factor requirements and interactions with the translation apparatus.
Nucleic acids research - 20 Apr 2016
Asnani Mukta, Pestova Tatyana V, Hellen Christopher U T
Abstract excerpt
Cadicivirus (CDV) is unique amongst picornaviruses in having a dicistronic genome with internal ribosomal entry sites (IRESs) preceding both open reading frames. Here, we investigated initiation on the 5'-terminal IRES. We report that the 982-nt long 5'UTR comprises 12 domains (d1-d12), five of which (d8-d12, nts 341-950) constitute a divergent Type I IRES. It comprises central elements (the apex of d10, d11 and...
Topics
- 5' Untranslated Regions
- Eukaryotic Initiation Factor-3
- Eukaryotic Initiation Factor-4A
- Eukaryotic Initiation Factor-4G
- Mutation
- Peptide Chain Initiation, Translational
- Picornaviridae
- RNA, Viral
- RNA-Binding Proteins
- Ribosome Subunits, Small, Eukaryotic
- Ribosomes