Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing.

doi:10.1038/srep20944

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Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing.

Scientific reports - 11 Feb 2016

Bolognesi Chiara, Forcato Claudio, Buson Genny, Fontana Francesca, Mangano Chiara, Doffini Anna, Sero Valeria, Lanzellotto Rossana, Signorini Giulio, Calanca Alex, Sergio Maximilian, Romano Rita, Gianni Stefano, Medoro Gianni, Giorgini Giuseppe, Morreau Hans, Barberis Massimo, Corver Willem E, Manaresi Nicolò

Abstract excerpt

Precision medicine in oncology requires an accurate characterization of a tumor molecular profile for patient stratification. Though targeted deep sequencing is an effective tool to detect the presence of somatic sequence variants, a significant number of patient specimens do not meet the requirements needed for routine clinical application. Analysis is hindered by contamination of normal cells and inherent tumor...

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PMID: 26864208
DOI: 10.1038/srep20944
PMCID: PMC4750064

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