Article
[Target-resequencing to identify microRNA-associated SNP and predict the effect of SNP on microRNA function in colorectal cancer patients].
Zhonghua zhong liu za zhi [Chinese journal of oncology] - 1 Oct 2015
Qiu Qingchong, Liu Jie, Shao Jiaofang, Lou Xiaoyan, Chen Chen, Lin Biaoyang
Abstract excerpt
OBJECTIVE: To identify SNPs in the miRNA genes in colorectal cancer (CRC) patients and to investigate their association with CRC. METHODS: DNAs were isolated from 30 CRC tumor tissues and 30 tumor-adjacent tissues, and subjected to target capture using a custom miRNA chip covering 685 miRNA genes from NimbleGen. The captured DNAs were then sequenced using the Illumina's sequencing technology, and the data were...
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