Article
Enhanced thermostability of mesophilic endoglucanase Z with a high catalytic activity at active temperatures.
International journal of biological macromolecules - 1 May 2016
Kim Su Jung, Joo Ji Eun, Jeon Sang Duck, Hyeon Jeong Eun, Kim Seung Wook, Um Young Soon, Han Sung Ok
Abstract excerpt
This is the first study for therrmostable mutants of mesophilic endoglucanase EngZ from Clostridium cellulovorans using by site-directed mutagenesis. K94R, S365P and their double mutant K94R/S365P had a wide range of active temperatures (30-60 °C). In addition, the optimal temperature of K94R/S365P was increased by 7.5 °C. K94R/S365P retained 78.3% relative activity at 70 °C, while the wild type retained only...
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