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Article

A Dicer-Independent Route for Biogenesis of siRNAs that Direct DNA Methylation in Arabidopsis.

Molecular cell - 21 Jan 2016

Ye Ruiqiang, Chen Zulong, Lian Bi, Rowley M Jordan, Xia Ning, Chai Jijie, Li Yan, He Xin-Jian, Wierzbicki Andrzej T, Qi Yijun

Abstract excerpt

DNA methylation directed by 24-nucleotide (nt) small interfering RNAs (siRNAs) plays critical roles in gene regulation and transposon silencing in Arabidopsis. 24-nt siRNAs are known to be processed from double-stranded RNAs by Dicer-like 3 (DCL3) and loaded into the effector Argonaute 4 (AGO4). Here we report a distinct class of siRNAs independent of DCLs (sidRNAs). sidRNAs are present as ladders of ∼ 20-60 nt...

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Identifiers

PMID: 26711010
DOI: 10.1016/j.molcel.2015.11.015
PMCID: PMC5110219

Genes

AGO4

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