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Article

Overestimation and underestimation of hepatitis C virus RNA levels in a widely used real-time polymerase chain reaction-based method.

Hepatology (Baltimore, Md.) - 1 Jul 2007

Chevaliez Stéphane, Bouvier-Alias Magali, Brillet Rozenn, Pawlotsky Jean-Michel

Abstract excerpt

UNLABELLED: The quantification of hepatitis C virus (HCV) RNA is essential for the everyday management of chronic hepatitis C therapy. Real-time polymerase chain reaction (PCR) techniques are potentially more sensitive than classical PCR techniques, are not prone to carryover contamination, and have a consistently wider dynamic range of quantification. Thus, they are rapidly replacing other technologies for the...

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PMID: 17525931
DOI: 10.1002/hep.21656

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