Article
5'-secondary structure formation, in contrast to a short string of non-preferred codons, inhibits the translation of the pyruvate kinase mRNA in yeast.
Yeast (Chichester, England) - 1 Jan 2000
Bettany A J, Moore P A, Cafferkey R, Bell L D, Goodey A R, Carter B L, Brown A J
Abstract excerpt
The effects of poor codon bias and secondary structure formation upon the translation of the pyruvate kinase (PYK1) mRNA have been investigated in Saccharomyces cerevisiae. Following insertion mutagenesis at the 5'-end of the PYK1 coding region, the gene was transformed into yeast, and translatio...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Codon
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Protein Biosynthesis
- Pyruvate Kinase
- RNA Probes
- RNA, Fungal
- RNA, Messenger
- Saccharomyces cerevisiae
- Transformation, Genetic