Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis.

doi:10.1002/jcp.25266

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Article

Establishment of Immortalized BMP2/4 Double Knock-Out Osteoblastic Cells Is Essential for Study of Osteoblast Growth, Differentiation, and Osteogenesis.

Journal of cellular physiology - 1 Jun 2016

Wu Li-An, Wang Feng, Donly Kevin J, Baker Andrew, Wan Chunyan, Luo Daoshu, MacDougall Mary, Chen Shuo

Abstract excerpt

Bone morphogenetic proteins 2 and 4 (BMP2/4) are essential for osteoblast differentiation and osteogenesis. Generation of a BMP2/4 dual knock-out ((ko/ko)) osteoblastic cell line is a valuable asset for studying effects of BMP2/4 on skeletal development. In this study, our goal was to create immortalized mouse deleted BMP2/4 osteoblasts by infecting adenoviruses with Cre recombinase and green fluorescent protein...

Topics

Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Cell Differentiation
Cell Line
Cell Proliferation
Cell Size
Extracellular Matrix
G1 Phase Cell Cycle Checkpoints
G2 Phase Cell Cycle Checkpoints

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Gene Expression Regulation

Gene Knockdown Techniques

Green Fluorescent Proteins

Integrases

Matrix Metalloproteinase 2

Matrix Metalloproteinase 9

Mice

Osteoblasts

Source

Open in PubMed Open full text

Identifiers

PMID: 26595646
DOI: 10.1002/jcp.25266
PMCID: PMC4784166

Genes

BMP2
BMP4

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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