Article
Single-Molecule Localization Microscopy allows for the analysis of cancer metastasis-specific miRNA distribution on the nanoscale.
Oncotarget - 29 Dec 2015
Oleksiuk Olga, Abba Mohammed, Tezcan Kerem Can, Schaufler Wladimir, Bestvater Felix, Patil Nitin, Birk Udo, Hafner Mathias, Altevogt Peter, Cremer Christoph, Allgayer Heike
Abstract excerpt
We describe a novel approach for the detection of small non-coding RNAs in single cells by Single-Molecule Localization Microscopy (SMLM). We used a modified SMLM-setup and applied this instrument in a first proof-of-principle concept to human cancer cell lines. Our method is able to visualize single microRNA (miR)-molecules in fixed cells with a localization accuracy of 10-15 nm, and is able to quantify and...
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