Article
Cell Lysis in S. pombe ura4 Mutants Is Suppressed by Loss of Functional Pub1, Which Regulates the Uracil Transporter Fur4.
PloS one - 1 Jan 2015
Nishino Kohei, Kushima Misaki, Matsuo Yuzy, Matsuo Yasuhiro, Kawamukai Makoto
Abstract excerpt
Schizosaccharomyces pombe Δura4 cells lyse when grown on YPD medium. A S. pombe non-essential gene deletion library was screened to determine suppressors of the lysis phenotype. Deletion of the pub1 gene, which encoded E3 ubiquitin ligase, strongly suppressed cell lysis in Δura4 cells. The Δpub1 cells displayed high sensitivity to 5-fluorouracil, a toxic analog of uracil, and this sensitivity was suppressed by...
Topics
- Carbon-Nitrogen Ligases
- Cell Membrane
- Chromatography, High Pressure Liquid
- Down-Regulation
- Fluorouracil
- Gene Deletion
- Golgi Apparatus
- Mass Spectrometry
- Microscopy, Fluorescence
- Mutation
- Schizosaccharomyces
- Schizosaccharomyces pombe Proteins
- Ubiquitination
- Uracil