Article
Polypeptone induces dramatic cell lysis in ura4 deletion mutants of fission yeast.
PloS one - 1 Jan 2013
Matsuo Yuzy, Nishino Kouhei, Mizuno Kouhei, Akihiro Takashi, Toda Takashi, Matsuo Yasuhiro, Kaino Tomohiro, Kawamukai Makoto
Abstract excerpt
Polypeptone is widely excluded from Schizosaccharomyces pombe growth medium. However, the reasons why polypeptone should be avoided have not been documented. Polypeptone dramatically induced cell lysis in the ura4 deletion mutant when cells approached the stationary growth phase, and this phenotype was suppressed by supplementation of uracil. To determine the specificity of this cell lysis phenotype, we created...
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