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Article

Improving a Synechocystis-based photoautotrophic chassis through systematic genome mapping and validation of neutral sites.

DNA research : an international journal for rapid publication of reports on genes and genomes - 1 Dec 2015

Pinto Filipe, Pacheco Catarina C, Oliveira Paulo, Montagud Arnau, Landels Andrew, Couto Narciso, Wright Phillip C, Urchueguía Javier F, Tamagnini Paula

Abstract excerpt

The use of microorganisms as cell factories frequently requires extensive molecular manipulation. Therefore, the identification of genomic neutral sites for the stable integration of ectopic DNA is required to ensure a successful outcome. Here we describe the genome mapping and validation of five neutral sites in the chromosome of Synechocystis sp. PCC 6803, foreseeing the use of this cyanobacterium as a...

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Identifiers

PMID: 26490728
DOI: 10.1093/dnares/dsv024
PMCID: PMC4675711

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