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A novel counter-selection method for markerless genetic modification in Synechocystis sp. PCC 6803.

Biotechnology progress - 1 Jan 2000

Cheah Yi Ern, Albers Stevan C, Peebles Christie A M

Abstract excerpt

The cyanobacterium Synechocystis sp. PCC 6803 is a photosynthetic organism capable of efficient harnessing of solar energy while capturing CO(2) from the environment. Methods to genetically alter its genomic DNA are essential for elucidating gene functions and are useful tools for metabolic engineering. In this study, a novel counter-selection method for the genetic alteration of Synechocystis was developed. This...

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DNA, Bacterial
DNA-Binding Proteins
Escherichia coli
Escherichia coli Proteins
Genetic Engineering

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Genetic Variation

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PMID: 23124993
DOI: 10.1002/btpr.1661

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