Article
Metabolite Profiling of Soybean Seed Extracts from Near-Isogenic Low and Normal Phytate Lines Using Orthogonal Separation Strategies.
Journal of agricultural and food chemistry - 11 Nov 2015
Jervis Judith, Kastl Christin, Hildreth Sherry B, Biyashev Ruslan, Grabau Elizabeth A, Saghai-Maroof Mohammad A, Helm Richard F
Abstract excerpt
Untargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS) was applied to lipid-depleted methanolic extracts of soybean seeds utilizing orthogonal chromatographic separations (reversed-phase and hydrophilic interaction) in both positive and negative ionization modes. Four near-isogenic lines (NILs) differing in mutations for two genes encoding highly homologous multidrug resistant...
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