Temperature-independent Detection of Heteroduplex and Homoduplex Fragments Applying Poly(glycidylmethacryate-co-divinylbenzene-based) Monoliths Modified to Strong Anion-exchanger.

Abstract excerpt

Monoliths of poly(glycidylmethacrylate-co-divinylbenzene) were prepared in the confines of presilanized borosilicate glass columns (100 × 3 mm I.D.). These monoliths were surface modified into strong anion-exchangers with hydrochloric acid (10%) and triethylamine, successively. The strong anion-exchanger established good separation of 5-phosphorylated oligodeoxythymidylic acids fragments [d(pT)(12-18)]. Moreover,...

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