Article
Expression, Purification, and Screening of BamE, a Component of the BAM Complex, for Structural Characterization.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Jeeves Mark, Sridhar Pooja, Knowles Timothy J
Abstract excerpt
In Gram-negative bacteria, integral outer membrane β-barrel proteins (OMP) are assembled by the β-barrel assembly machine complex, or BAM complex. This complex includes the essential components BamA, an OMP composed of a carboxyl terminal β-barrel domain and five polypeptide transport-associated domains (POTRA), and the lipoprotein BamD. In Escherichia coli, the complex contains an additional three lipoproteins,...
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