Spontaneous and on point: Do spontaneous mutations used for laboratory experiments cause pleiotropic effects that might confound bacterial infection and evolution assays?

Abstract excerpt

Many selectable phenotypes in microbial systems, including antibiotic resistance, can be conferred by single point mutations. This is frequently exploited in research, where the selection and use of microbial mutants that are spontaneously resistant to antibiotics like rifampicin and streptomycin facilitate the recovery and/or quantification of a target microbe. Such mutations are commonly employed as genetic...

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