Article
Differentiation of Campylobacter jejuni and Campylobacter coli Using Multiplex-PCR and High Resolution Melt Curve Analysis.
PloS one - 1 Jan 2015
Banowary Banya, Dang Van Tuan, Sarker Subir, Connolly Joanne H, Chenu Jeremy, Groves Peter, Ayton Michelle, Raidal Shane, Devi Aruna, Vanniasinkam Thiru, Ghorashi Seyed A
Abstract excerpt
Campylobacter spp. are important causes of bacterial gastroenteritis in humans in developed countries. Among Campylobacter spp. Campylobacter jejuni (C. jejuni) and C. coli are the most common causes of human infection. In this study, a multiplex PCR (mPCR) and high resolution melt (HRM) curve analysis were optimized for simultaneous detection and differentiation of C. jejuni and C. coli isolates. A segment of...
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