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Rapid Identification of <i>Campylobacter</i> spp. by Melting Peak Analysis of Biprobes in Real-Time PCR

1 Jun 2001

Abstract excerpt

We describe rapid PCR-biprobe identification of Campylobacter spp. This is based on real-time PCR with product analysis in the same system. The assay identifies enteropathogenic campylobacters to the species level on the basis of their degree of hybridization to three 16S ribosomal DNA (rDNA) biprobes. First-round symmetric PCR is performed with genus-specific primers which selectively target and amplify a...

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PMID: 11376061
DOI: 10.1128/jcm.39.6.2227-2232.2001

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