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Article

Campylobacter jejuni and Campylobacter coli genotyping by high-resolution melting analysis of a flaA fragment.

Applied and environmental microbiology - 1 Jan 2010

Merchant-Patel Shreema, Blackall Patrick J, Templeton Jillian, Price Erin P, Tong Steven Y C, Huygens Flavia, Giffard Philip M

Abstract excerpt

The highly variable flagellin-encoding flaA gene has long been used for genotyping Campylobacter jejuni and Campylobacter coli. High-resolution melting (HRM) analysis is emerging as an efficient and robust method for discriminating DNA sequence variants. The objective of this study was to apply HRM analysis to flaA-based genotyping. The initial aim was to identify a suitable flaA fragment. It was found that the...

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Identifiers

PMID: 19933351
DOI: 10.1128/AEM.01164-09
PMCID: PMC2805211

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Source receipt: pubmed, retrieved 7 Sept 2026.

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