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Article

High-resolution melting system to perform multilocus sequence typing of Campylobacter jejuni.

PloS one - 24 Jan 2011

Lévesque Simon, Michaud Sophie, Arbeit Robert D, Frost Eric H

Abstract excerpt

Multi-locus sequence typing (MLST) has emerged as the state-of-the-art method for resolving bacterial population genetics but it is expensive and time consuming. We evaluated the potential of high resolution melting (HRM) to identify known MLST alleles of Campylobacter jejuni at reduced cost and time. Each MLST locus was amplified in two or three sub fragments, which were analyzed by HRM. The approach was...

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Identifiers

PMID: 21297862
DOI: 10.1371/journal.pone.0016167
PMCID: PMC3026018

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