Article
Evaluation of PCR and high-resolution melt curve analysis for differentiation of Salmonella isolates.
Avian pathology : journal of the W.V.P.A - 1 Jun 2017
Saeidabadi Mohammad Sadegh, Nili Hassan, Dadras Habibollah, Sharifiyazdi Hassan, Connolly Joanne, Valcanis Mary, Raidal Shane, Ghorashi Seyed Ali
Abstract excerpt
Consumption of poultry products contaminated with Salmonella is one of the major causes of foodborne diseases worldwide and therefore detection and differentiation of Salmonella spp. in poultry is important. In this study, oligonucleotide primers were designed from hemD gene and a PCR followed by high-resolution melt (HRM) curve analysis was developed for rapid differentiation of Salmonella isolates. Amplicons of...
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