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Nucleated red blood cells impact DNA methylation and expression analyses of cord blood hematopoietic cells

11 Sept 2015

Abstract excerpt

BACKGROUND: Genome-wide DNA methylation (DNAm) studies have proven extremely useful to understand human hematopoiesis. Due to their active DNA content, nucleated red blood cells (nRBCs) contribute to epigenetic and transcriptomic studies derived from whole cord blood. Genomic studies of cord blood hematopoietic cells isolated by fluorescence-activated cell sorting (FACS) may be significantly altered by...

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PMID: 26366232
DOI: 10.1186/s13148-015-0129-6

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