Article
Altering the orientation of a fused protein to the RNA-binding ribosomal protein L7Ae and its derivatives through circular permutation.
Biochemical and biophysical research communications - 23 Oct 2015
Ohuchi Shoji J, Sagawa Fumihiko, Sakamoto Taiichi, Inoue Tan
Abstract excerpt
RNA-protein complexes (RNPs) are useful for constructing functional nano-objects because a variety of functional proteins can be displayed on a designed RNA scaffold. Here, we report circular permutations of an RNA-binding protein L7Ae based on the three-dimensional structure information to alter the orientation of the displayed proteins on the RNA scaffold. An electrophoretic mobility shift assay and atomic...
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