Article
Screening for recombinants of Crambe abyssynica after transformation by the pMF1 marker-free vector based on chemical selection and meristematic regeneration.
Scientific reports - 11 Sept 2015
Qi Weicong, Tinnenbroek-Capel Iris E M, Salentijn Elma M J, Schaart Jan G, Cheng Jihua, Denneboom Christel, Zhang Zhao, Zhang Xiaolin, Zhao Han, Visser Richard G F, Huang Bangquan, Van Loo Eibertus N, Krens Frans A
Abstract excerpt
The T-DNA region of pMF1 vector of marker-free system developed by Wageningen UR, has Recombinase R-LBD gene fusion and nptII and codA gene fusion between two recombination sites. After transformation applying dexamethasone (DEX) can activate the recombinase to remove the T-DNA fragment between recombination sites. The recombinant ought to be selected on 5-fluorocytocine (5-FC) because of codA converting 5-FC...
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