Article
A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency.
The Plant journal : for cell and molecular biology - 1 Jun 2000
Sugita K, Kasahara T, Matsunaga E, Ebinuma H
Abstract excerpt
We represent here the GST-MAT vector system. The R recombinase gene of the site-specific recombination system R/RS from Zygosaccharomyces rouxii was fused to the chemical inducible promoter of the glutathione-S-transferase (GST-II-27) gene from Zea mays. Upon excision, the isopentenyltransferase (ipt) gene that is used as a selectable marker gene is removed. When the cauliflower mosaic virus 35S promoter (CaMV...
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