Article
Stable transformation and direct regeneration in Coffea canephora P ex. Fr. by Agrobacterium rhizogenes mediated transformation without hairy-root phenotype.
Plant cell reports - 1 Mar 2006
Kumar Vinod, Satyanarayana K V, Sarala Itty S, Indu E P, Giridhar P, Chandrashekar A, Ravishankar G A
Abstract excerpt
A system for genetic transformation of Coffea canephora by co-cultivation with Agrobacterium rhizogenes harbouring a binary vector has been developed. The objective of the present study was the genetic transformation and direct regeneration of transformants through secondary embryos bypassing an intervening hairy root stage. Transformants were obtained with a transformation efficiency up to 3% depending on the...
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