Article
A transformation method for obtaining marker-free plants of a cross-pollinating and vegetatively propagated crop.
Nature biotechnology - 1 Apr 2003
de Vetten Nick, Wolters Anne-Marie, Raemakers Krit, van der Meer Ingrid, ter Stege Renaldo, Heeres Els, Heeres Paul, Visser Richard
Abstract excerpt
It is generally thought that transformation of plant cells using Agrobacterium tumefaciens occurs at a very low frequency. Therefore, selection marker genes are used to identify the rare plants that have taken up foreign DNA. Genes encoding antibiotic and herbicide resistance are widely used for this purpose in plant transformation. Over the past several years, consumer and environmental groups have expressed...
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