Article
A versatile and efficient markerless gene disruption system for Acidithiobacillus thiooxidans: application for characterizing a copper tolerance related multicopper oxidase gene.
Environmental microbiology - 1 Nov 2014
Wen Qing, Liu Xiangmei, Wang Huiyan, Lin Jianqun
Abstract excerpt
The acidophilic bioleaching bacteria can usually survive in high concentrations of copper ions because of their special living environment. However, little is known about the copper homeostatic mechanisms of Acidithiobacillus thiooxidans, an important member of bioleaching bacteria. Here, a putative multicopper oxidase gene (cueO) was detected from the draft genome of A. thiooxidans ATCC 19377. The...
Topics
- Acidithiobacillus thiooxidans
- Copper
- Gene Knockout Techniques
- Molecular Sequence Data
- Monophenol Monooxygenase
- Mutation
- Oxidoreductases
- beta-Galactosidase