Article
E6 and E7 gene silencing results in decreased methylation of tumor suppressor genes and induces phenotype transformation of human cervical carcinoma cell lines.
Oncotarget - 15 Sept 2015
Li Liming, Xu Cui, Long Jia, Shen Danbei, Zhou Wuqing, Zhou Qiyan, Yang Jia, Jiang Mingjun
Abstract excerpt
In SiHa and CaSki cells, E6 and E7-targeting shRNA specifically and effectively knocked down human papillomavirus (HPV) 16 E6 and E7 at the transcriptional level, reduced the E6 and E7 mRNA levels by more than 80% compared with control cells that expressed a scrambled-sequence shRNA. E6 and E7 repression resulted in down-regulation of DNA methyltransferase mRNA and protein expression, decreased DNA methylation...
Topics
- Apoptosis
- Carcinoma
- Cell Line, Tumor
- Cell Proliferation
- Cell Survival
- DNA (Cytosine-5-)-Methyltransferases
- DNA Methylation
- Down-Regulation
- Female
- Gene Expression Profiling